BiocenterInnsbruck Medical University



BIOINFORMATICS EXERCISE 3



NOTES

INTRODUCTION (EX3)
CHEAT SHEET (EX3)
  

1 CLONING EXPERIMENT

1.1Starting from a given cloning sequence and find out which cloning vector (name, accnr, length) was used (e.g. using the tool VecScreen).
1.2Where is the insert in the given sequence located (position from-to, length)?
1.3Identify the insert (name, accnr, organism, length, amino acid sequence, function)?
1.4Determine the restriction enzyme (6bp cutter) used for incorporating the insert (name, sequence).
1.5Are there homolog proteins in humans (name, accnr, description, conserved domains)
  

2 PROTEIN 3D-STRUCTURE

2.1Show 3D protein structure of a homolog human protein (including DNA binding) (from 1.5) and give PDB identification code for this structure
  

LINKS

 BLAST
 VecScreen
 Restriction enzyme digest of DNA (insilico)
 Conserved Domain Database (CDD)
 PDB
 Proteopedia
 POLYVIEW-3D
 JMol
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